重组Anti-CD3G抗体[EPR4516] (ab108996)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4516] to CD3G
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD3G抗体[EPR4516]
参阅全部 CD3G 一抗 -
描述
兔单克隆抗体[EPR4516] to CD3G -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human tonsil, Human fetal thymus, HuT-78 and Jurkat whole cell lysate (ab7899); Human tonsil tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4516 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108996于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 20 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
靶标
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功能
The CD3 complex mediates signal transduction. -
序列相似性
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
结构域
A di-leucine motif and a tyrosine-based motif are individually sufficient to induce both endocytosis and delivery to lysosomes. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 917 Human
- Omim: 186740 Human
- SwissProt: P09693 Human
- Unigene: 2259 Human
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别名
- CD3 gamma antibody
- CD3-GAMMA antibody
- CD3g antibody
see all
图片
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All lanes : Anti-CD3G antibody [EPR4516] (ab108996) at 1/1000 dilution
Lane 1 : Human tonsil tissue lysate
Lane 2 : Human fetal thymus tissue lysate
Lane 3 : HuT-78 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 20 kDa -
Anti-CD3G antibody [EPR4516] (ab108996) at 1/1000 dilution + Jurkat cell lysate at 10 µg
Predicted band size: 20 kDa -
ab108996, at 1/100 dilution, staining CD3G in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab108996 被引用在 2 文献中.
- Blaszkiewicz M et al. The involvement of neuroimmune cells in adipose innervation. Mol Med 26:126 (2020). PubMed: 33297933
- Pérez-Mazliah D et al. Follicular Helper T Cells are Essential for the Elimination of Plasmodium Infection. EBioMedicine 24:216-230 (2017). PubMed: 28888925