Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mammary tumor)
Permeabilization
No
Specification
mammary tumor
Blocking step
ab64261 kit as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Acetone
Other product details
Incubation time
10 minute(s)
Secondary antibody
Name
Abcam antibody:Rabbit specific HRP/DAB (ABC) Detection IHC Kit
Additional data
Additional Notes
Sections were fixed in acetone 5 min, washed in 0.2%Tween PBS and processed using instructions and reagents from the "rabbit-specific HRP/DAB Detection Kit" ab64261. Sections were counterstained with Mayer's hematoxylin. Overall, staining is "inconclusive". CD36 can be expressed by many cell types, which probably contributes to its wide distribution withing the tumor.
Abcam response
We have not tested this on sections of frozen tissue. In general, we suggest testing a panel of fixatives to determine which is most compatible with the antibody, for example acetone, acetone:ethyl alcohol 50:50 mixture, and 4% PFA.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Sep 12 2017