Application
Flow Cytometry
Sample
Human Cell (KG-1 cells (human acute myeloid leukemia cell line)
Specification
KG-1 cells (human acute myeloid leukemia cell line
Preparation
Cell harvesting/tissue preparation method: Cells grown in suspension in RPMI-1640 and centrifuged before staining
Sample buffer: FACS buffer (PBS 0.5% FCS 0.5M EDTA)
Sample buffer: FACS buffer (PBS 0.5% FCS 0.5M EDTA)
Fixation
None
Permeabilization
No
Gating Strategy
All live cells
Other product details
Incubation time
20 minute(s) · Temperature: 4°C · Diluent: FACS buffer (PBS 0.5% FCS 0.5M EDTA)
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Antibody can not be diluted and is supplied at a low concentration, therefore recommend that antibody is applied to a cell pellet in minimum buffer to achieve best staining (10 ul added to a cell pellet re-suspended in a minimum volume of buffer).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Mar 01 2010