重组Anti-CD32B抗体[EP888Y] (ab45143)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP888Y] to CD32B
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD32B抗体[EP888Y]
参阅全部 CD32B 一抗 -
描述
兔单克隆抗体[EP888Y] to CD32B -
宿主
Rabbit -
特异性
This antibody has weak cross-reactivity with CD32A and CD32C. It detects weak signal in CD32A and CD32C over-expressed lysates at the dilution of 1/200 under the exposure time of 3 minutes, but detects strong band in CD32B over-expressed lysates at the dilution of 1:2000 under the exposure time of 15 seconds. -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human CD32B aa 250-350 (C terminal).
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阳性对照
- Daudi cell lysate, human lymph node tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP888Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab45143于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/10000 - 1/50000. Detects a band of approximately 49-55 kDa.
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IHC-P |
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
1/10000 - 1/50000. Detects a band of approximately 49-55 kDa. |
IHC-P
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis. -
组织特异性
Is the most broadly distributed Fc-gamma-receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells. -
疾病相关
Note=A chromosomal aberration involving FCGR2B is found in a follicular lymphoma. Translocation t(1;22)(q22;q11). The translocation leads to the hyperexpression of the receptor. This may play a role in the tumor progression. -
序列相似性
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
结构域
Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2213 Human
- Omim: 604590 Human
- SwissProt: P31994 Human
- Unigene: 654395 Human
- Unigene: 713617 Human
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别名
- CD32 antibody
- CD32 antigen antibody
- CD32B antibody
see all
图片
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Unpurified ab45143 staining human CD32 (1/100) in human lymph node by immunohistochemistry using paraffin embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-CD32B antibody [EP888Y] (ab45143) at 1/10000 dilution (purified)
Lane 1 : Human thyroid lysate
Lane 2 : Daudi whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Observed band size: 40-44 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
Immunohistochemical staining of paraffin embedded human spleen with purified ab45143 at a working dilution of 1/1000. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Lanes 1-2 & 5-6 : Anti-CD32B antibody [EP888Y] (ab45143) at 1/200 dilution (unpurified)
Lanes 3-4 : Anti-CD32B antibody [EP888Y] (ab45143) at 1/2000 dilution (unpurified)
Lane 1 : Human CD32A overexpressed lysate
Lanes 2 & 4 & 6 : Non-transfected lysate
Lane 3 : Human CD32B overexpressed lysate
Lane 5 : Human CD32C overexpressed lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 39 kDa why is the actual band size different from the predicted?Exposure times: Lanes 1 and 2: 3 minutes, Lanes 3 and 4: 15 seconds, Lanes 5 and 6: 3 minutes.
Blocking and dilution buffer: 5% NFDM/TBST.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (19)
ab45143 被引用在 19 文献中.
- Hu C et al. Dihydroarteannuin Ameliorates Collagen-Induced Arthritis Via Inhibiting B Cell Activation by Activating the FcγRIIb/Lyn/SHP-1 Pathway. Front Pharmacol 13:883835 (2022). PubMed: 35592412
- Gwon Y et al. Amelioration of amyloid ß-Fc?RIIb neurotoxicity and tau pathologies by targeting LYN. FASEB J 33:4300-4313 (2019). PubMed: 30540497
- Roghanian A et al. Cyclophosphamide Enhances Cancer Antibody Immunotherapy in the Resistant Bone Marrow Niche by Modulating Macrophage FcγR Expression. Cancer Immunol Res 7:1876-1890 (2019). PubMed: 31451483
- Shang L et al. Vaccine-Associated Maintenance of Epithelial Integrity Correlated With Protection Against Virus Entry. J Infect Dis 218:1272-1283 (2018). PubMed: 29401315
- Arthur SE et al. Genome-wide discovery of somatic regulatory variants in diffuse large B-cell lymphoma. Nat Commun 9:4001 (2018). PubMed: 30275490