Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Permeabilization
Yes - 0.3% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Other product details
Dilution
1/200
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluro 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
Additional data
Additional Notes
Human brain section stained with anti-CD31 (ab32457) and secondary antibody Alexa 488. Antibody recognise endothelial cell and blood vessels of adult brain.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ruma Raha-Chowdhury
Verified customer
提交于 May 11 2012