Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Breast tumors)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TE Buffer, pH 9
Permeabilization
Yes - 0.3% Triton-X/PBS for 5min
Specification
Breast tumors
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking Buffer
Dilution
1/200
Secondary antibody
Dilution
1/250
Name
Non-Abcam antibody was used: donkey anti-rabbit Alexa647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 21 2017