Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Left ventricle)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Trilogy
Permeabilization
Yes - Trilogy
Specification
Left ventricle
Blocking step
Dako Envision Kit 4007 (Block) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
10% Formalin
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS-T (0.5%)
Secondary antibody
Name
Non-Abcam antibody was used: DAKO envision Kit (4007)
Host species: Rabbit
Clonality: Monoclonal
Conjugation: HRP polymer
Host species: Rabbit
Clonality: Monoclonal
Conjugation: HRP polymer
Additional data
Abcam response
We would like to thank this researcher for submitting their review. This antibody had not been tested for cross reactivity with rat and we were unsure of what the results would be. The reviewer received an AbTrial discount code for using the antibody in an untested species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Mar 16 2017