Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Fish Tissue sections (Heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citrate pH6.0
Permeabilization
Yes - Triton X
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA 1% in PBS
Dilution
1/100
Secondary antibody
Dilution
1/100
Name
Non-Abcam antibody was used: Goat Anti Mouse IgG (H&L) peroxidase conjugated (A
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Peroxidase Substrate: DAB-Ni solution 0.2% with H2O2
Results: No staining
Abcam response
Thank you for submitting this Abreview. As this antibody hasn't been validated for fish samples, we are unsure if the antibody is suitable for this species. It may require further optimization.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Dec 24 2014