Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Skin)
Permeabilization
No
Specification
Skin
Blocking step
(agent) for 10 minute(s) · Concentration: 0.25% · Temperature: RT°C
Fixative
Acetone
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Diluent: 1% FBS in TBS
Dilution
1/50
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Anti-rabbit IgG, Molecular Probe
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Additional data
Additional Notes
This antibody picked up only a subset of vessels when we stained in human skin
Abcam response
The antibody has not been tested in IHC-Fr by Abcam and for now it is not guaranteed in this application. However, if that would be of interest, further Abtrial testing attempts are welcomed.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Oct 21 2015