重组Anti-CD3 epsilon抗体[E272] (ab32186)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E272] to CD3 epsilon
- Suitable for: IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD3 epsilon抗体[E272]
参阅全部 CD3 epsilon 一抗 -
描述
兔单克隆抗体[E272] to CD3 epsilon -
宿主
Rabbit -
经测试应用
适用于: IHC-P, IPmore details
不适用于: Flow Cyt or WB -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human CD3 epsilon aa 150-250 (C terminal). The exact sequence is proprietary.
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阳性对照
- IP: Jurkat cell lysate IHC-P: Human lymph node.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
E272 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32186于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
1/50.
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说明 |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
1/50. |
靶标
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功能
The CD3 complex mediates signal transduction. -
序列相似性
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 916 Human
- Omim: 186830 Human
- SwissProt: P07766 Human
- Unigene: 3003 Human
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别名
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
see all
图片
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Ab32186, at a 1/100 dilution, staining CD3 epsilon in paraffin embedded human lymph node tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot analysis of immunoprecipitation pellets from Jurkat cell lysate or PBS (lane 2: negative control), using ab32186 at a 1/50 dilution. The predicted band size of 23 kDa was observed.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab32186 尚未被引用在任何文献中。