Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 100C 15min in 10 mM Sodium Citrate Buffer (pH 6.0), cooled for 30min, then used 3% H2O2 for 10min
Sample
Mouse Tissue sections (Pancreas)
Specification
Pancreas
Permeabilization
No
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: SignalStain Antibody Diluent #8112
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: SignalStain Boost IHC Detection Reagent (HRP, Rabb
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
antigen unmasking: boiling the slides for 15min in 10 mM Sodium Citrate Buffer (pH 6.0), cooled for 30min, then used 3% H2O2 for 10min.
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提交于 Jul 23 2013