Application
Flow Cytometry
Sample
Human Cell (PBMC)
Specification
PBMC
Preparation
Cell harvesting/tissue preparation method: from buffy coat
Sample buffer: PBS/BSA 2% sodium azide 0.01%
Sample buffer: PBS/BSA 2% sodium azide 0.01%
Permeabilization
No
Gating Strategy
lymphocytes
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS/BSA 2% sodium azide 0.01%
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
No fixation step was done.
Gating was done on CD19+ B cells: CD19+ B cells are stained as CD3- and CD19- T cells (which should be T cells) are CD3+.
Gating was done on CD19+ B cells: CD19+ B cells are stained as CD3- and CD19- T cells (which should be T cells) are CD3+.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 15 2011