Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Trigeminal Ganglia)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH 6.0 + .05% Tween-20
Permeabilization
Yes - 0.5% Triton-X-100
Specification
Trigeminal Ganglia
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 1% Normal goat serum
Dilution
1/100
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondar
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Additional data
Additional Notes
Tissue fixed in freshly made 4% formaldehyde. HIER in kitchen pressure cooker, maintained above 100 degrees C for 5 mins, cooled in buffer, and washed with PBS+.05% Tween-20.
PubMed ID
29175626: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Kevin Egan
Verified customer
提交于 Mar 22 2018