Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (brain)
Specification
brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
BSA + serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/600
Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA + 5% NGS
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Dilution
1/200
Additional data
Additional Notes
I have additional images of the staining in several brain areas in various magnifications. Unfortunately i cant attach more then one file. Therefore if you are interested in receiving the images please inform me of your email address.
Best regards,
Best regards,
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 22 2012