Anti-CD20抗体[MEM-97] (ab8237)
Key features and details
- Mouse monoclonal [MEM-97] to CD20
- Suitable for: Flow Cyt, IP
- Reacts with: Dog, Human, Pig
- Isotype: IgG1
概述
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产品名称
Anti-CD20抗体[MEM-97]
参阅全部 CD20 一抗 -
描述
小鼠单克隆抗体[MEM-97] to CD20 -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, IPmore details -
种属反应性
与反应: Dog, Human, Pig -
免疫原
Tissue, cells or virus corresponding to Human CD20. Burkitt's lymphoma cell line Raji.
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常规说明
This product was changed from ascites to tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
Purified from TCS -
克隆
单克隆 -
克隆编号
MEM-97 -
同种型
IgG1 -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
Use a concentration of 1 - 5 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IP |
Use at an assay dependent concentration.
|
说明 |
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Flow Cyt
Use a concentration of 1 - 5 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IP
Use at an assay dependent concentration. |
靶标
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功能
This protein may be involved in the regulation of B-cell activation and proliferation. -
组织特异性
Expressed on B-cells. -
疾病相关
Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
序列相似性
Belongs to the MS4A family. -
翻译后修饰
Phosphorylated. Might be functionally regulated by protein kinase(s). -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 485430 Dog
- Entrez Gene: 931 Human
- Omim: 112210 Human
- SwissProt: Q3C2E2 Dog
- SwissProt: P11836 Human
- Unigene: 712553 Human
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别名
- APY antibody
- ATOPY antibody
- B lymphocyte antigen CD20 antibody
see all
图片
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Separation of human CD20 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab8237 (concentration in sample 1.6 μg/ml) GAM APC.
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Flow cytometry surface staining pattern of human peripheral whole blood stained using ab8237 (concentration in sample 1.6 μg/ml) GAM APC.
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Double Immunostaining of CD20 and CD79a in human, canine and porcine B lymphocytes using ab8237 and ab76908 by Flow Cytometery.
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Immunoprecipitation of human CD20 from the whole cell lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with anti-CD20 rabbit polyclonal antibody. Lane 1: original lysate of RAJI cells Lane 2: immunoprecipitate by Isotypic mouse IgG1 control Lane 3: original lysate of RAJI cells Lane 4: immunoprecipitate by anti-CD20 (MEM-97, ab 8237)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab8237 被引用在 3 文献中.
- Zhang GP et al. MicroRNA-98 regulates osteogenic differentiation of human bone mesenchymal stromal cells by targeting BMP2. J Cell Mol Med 21:254-264 (2017). Flow Cyt ; Human . PubMed: 27860183
- Qiao Y et al. A robust approach to enhance tumor-selective accumulation of nanoparticles. Oncotarget 2:59-68 (2011). Mouse . PubMed: 21378416
- Szollosi J et al. Supramolecular complexes of MHC class I, MHC class II, CD20, and tetraspan molecules (CD53, CD81, and CD82) at the surface of a B cell line JY. J Immunol 157:2939-46 (1996). PubMed: 8816400