Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Specification
skin
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH 8, 20 min, 100C
Permeabilization
No
Other product details
Dilution
1/200
Incubation time
20 minute(s) · Temperature: 25°C · Diluent: Dako diluent, background reducers
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse anti-rabbit IgG, Leica Refine
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Additional data
Additional Notes
Procedure automated on Leica Microsystems Bond III IHC system, 20 minute antibody incubation, using Refine Polymer detection. No permeabilization or blocking necessary, other than endogenous peroxidase with H2O2.
Numerous antigen retrieval regimes tested - EDTA gave best signal/background
Numerous antigen retrieval regimes tested - EDTA gave best signal/background
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Dec 12 2011