Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (kidney)
Specification
kidney
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate acid
Permeabilization
Yes - triton x-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Other product details
Dilution
1/50
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: tbst
Secondary antibody
Name
Non-Abcam antibody was used: vector elite abc
Host species: Mouse
Clonality: Monoclonal
Conjugation: Biotin
Host species: Mouse
Clonality: Monoclonal
Conjugation: Biotin
Dilution
1/1000
Additional data
Additional Notes
Could you please upload an image of your results?
Unpublished data, under revision.
How long was the fixation of the sheep kidney sections?
24 hours
Did you carry out a control on Human samples?
No, I have clear difference between controls and cases plus I used negative control
How long was the permeabilization with Triton-X?
30 minutes
Unpublished data, under revision.
How long was the fixation of the sheep kidney sections?
24 hours
Did you carry out a control on Human samples?
No, I have clear difference between controls and cases plus I used negative control
How long was the permeabilization with Triton-X?
30 minutes
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MRS. Mari Palviainen
Verified customer
提交于 Mar 04 2011