重组HRP Anti-CD166抗体[EPR2759(2)] (ab196846)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR2759(2)] to CD166
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
概述
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产品名称
HRP Anti-CD166抗体[EPR2759(2)]
参阅全部 CD166 一抗 -
描述
HRP兔单克隆抗体[EPR2759(2)] to CD166 -
宿主
Rabbit -
偶联物
HRP -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: SH-SY5Y whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2759(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab196846于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Detects a band of approximately 100-105 kDa (predicted molecular weight: 65 kDa).
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说明 |
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WB
1/1000. Detects a band of approximately 100-105 kDa (predicted molecular weight: 65 kDa). |
靶标
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功能
Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. -
组织特异性
Spleen, placenta, liver, and weakly in liver. Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. Expressed by neurons in the brain. Restricted expression in tumor cell lines. Preferentially expressed in highly metastasizing melanoma cell lines. -
序列相似性
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
结构域
The CD6 binding site is located in the N-terminal Ig-like domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 214 Human
- Entrez Gene: 11658 Mouse
- Entrez Gene: 79559 Rat
- Omim: 601662 Human
- SwissProt: Q13740 Human
- SwissProt: Q61490 Mouse
- SwissProt: O35112 Rat
- Unigene: 591293 Human
see all -
别名
- Activated leukocyte cell adhesion molecule antibody
- ALCAM antibody
- ALCAM protein antibody
see all
图片
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HRP Anti-CD166 antibody [EPR2759(2)] (ab196846) at 1/1000 dilution + SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 100-105 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab196846 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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