Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Lymph node)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
Lymph node
Blocking step
(agent) for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: Emerald diluent antibody, Cellmarque
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Ultratek HRP kit, ScyTek Laboratories, Logan, Utah
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
The antibody was at a dilution of 1:100, without antigen retrieval and with an heating antigen retrieval for 40 minutes at pH 4, pH6 and pH9. Strong cytoplasmic labelling in sinusoidal macrophages was observed at pH6 and 9, without any background noise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Sara Belluco
Verified customer
提交于 Sep 11 2017