The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at a concentration of 0.5 - 1 µg/ml. This concentration detects approximately 0.06 ng/well of Human CD137.
WB: Use at a concentration of 0.1 - 0.2 µg/ml. This concentration detects Human CD137 at approximately 0.2 ng/lane and 0.5 ng/lane under non-reducing and reducing conditions, respectively. Protein exists as a monomer of 30 kDa and a dimer of 55 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Receptor for TNFSF14/4-1BBL. Possibly active during T cell activation.