Anti-CD13抗体[R3-63] (ab33489)
Key features and details
- Rat monoclonal [R3-63] to CD13
- Suitable for: IHC-Fr, IHC-FrFl, Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2a
Related conjugates and formulations
概述
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产品名称
Anti-CD13抗体[R3-63]
参阅全部 CD13 一抗 -
描述
大鼠单克隆抗体[R3-63] to CD13 -
宿主
Rat -
特异性
ab33489 recognizes mouse aminopeptidase N (APN), a cell surface protein homologous with human CD13. -
经测试应用
适用于: IHC-Fr, IHC-FrFl, Flow Cytmore details -
种属反应性
与反应: Mouse -
免疫原
Intestinal CD13 (Mouse)
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常规说明
Clone R3-63 has been reported to block mouse APN enzyme activity.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified IgG prepared by affinity chromatography on Protein G. -
Primary antibody说明
Clone R3-63 has been reported to block mouse APN enzyme activity. -
克隆
单克隆 -
克隆编号
R3-63 -
骨髓瘤
IR983F -
同种型
IgG2a -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab33489于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr | (1) |
Use at an assay dependent concentration.
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IHC-FrFl |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
Use 10ul of the suggested working dilution to label 10^6 cells. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
说明 |
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IHC-Fr
Use at an assay dependent concentration. |
IHC-FrFl
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. Use 10ul of the suggested working dilution to label 10^6 cells. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection. -
组织特异性
Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients. -
序列相似性
Belongs to the peptidase M1 family. -
结构域
Amino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies). -
翻译后修饰
Sulfated.
N- and O-glycosylated.
May undergo proteolysis and give rise to a soluble form. -
细胞定位
Cell membrane. Cytoplasm > cytosol. A soluble form has also been detected. - Information by UniProt
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数据库链接
- Entrez Gene: 16790 Mouse
- SwissProt: P97449 Mouse
- Unigene: 4487 Mouse
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别名
- Alanyl (membrane) aminopeptidase antibody
- Alanyl aminopeptidase antibody
- Aminopeptidase M antibody
see all
实验方案
数据表及文件
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Datasheet download
文献 (6)
ab33489 被引用在 6 文献中.
- Delgado AC et al. Release of stem cells from quiescence reveals gliogenic domains in the adult mouse brain. Science 372:1205-1209 (2021). PubMed: 34112692
- Sugimura R et al. Biomimetic aorta-gonad-Mesonephros-on-a-Chip to study human developmental hematopoiesis. Biomed Microdevices 22:34 (2020). PubMed: 32377802
- Poropatich K et al. Elemental Zn and its Binding Protein Zinc-a2-Glycoprotein are Elevated in HPV-Positive Oropharyngeal Squamous Cell Carcinoma. Sci Rep 9:16965 (2019). PubMed: 31740720
- Luo X et al. Choroidal pericytes promote subretinal fibrosis after experimental photocoagulation. Dis Model Mech 11:N/A (2018). PubMed: 29622551
- Crouch EE et al. Regional and stage-specific effects of prospectively purified vascular cells on the adult V-SVZ neural stem cell lineage. J Neurosci 35:4528-39 (2015). PubMed: 25788671
- Hansen AS et al. A mouse aminopeptidase N is a marker for antigen-presenting cells and appears to be co-expressed with major histocompatibility complex class II molecules. Eur J Immunol 23:2358-64 (1993). PubMed: 8103749