Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (oral mucosa (tongue))
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: proteinase K
Permeabilization
No
Specification
oral mucosa (tongue)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: BSA 1%
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: LSAB kit (Dako)
Host species: Mouse
Clonality: Polyclonal
Conjugation: Biotin
Host species: Mouse
Clonality: Polyclonal
Conjugation: Biotin
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Luciana Correa
Verified customer
提交于 Jun 18 2015