Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (macaque retina tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6 citrate
Permeabilization
No
Specification
macaque retina tissue
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2.5% serum/PBS
Dilution
1/200
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: donkey anti-goat AlexaFluor 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 594
Additional data
Additional Notes
this antibody had extremely high nonspecific staining and reacted with many parts of the retina section that it should't have stained (nuclei, photoreceptors. etc)
Abcam response
We would like to thank the reviewer for submitting this Abreview for this product. As the product had not yet been tested in this application on this tissue type we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. We encourage all our customers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Mar 31 2017