Application
Flow Cytometry
Fixation
None
Permeabilization
No
Sample
Cow Cell (Peripheral blood)
Specification
Peripheral blood
Gating Strategy
Granulocytes
Preparation
Cell harvesting/tissue preparation method: Sodium citrate anticoagulated fresh blood
Sample buffer: None
Sample buffer: None
Other product details
Incubation time
30 minute(s) · Temperature: 4°C · Diluent: None
Dilution
1/6
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
20 ul antibody was used to stain 100 ul peripheral blood.
The blood was then lysed (BD Cell Lyse), followed by one wash with PBS with 5% sodium azide and 0.2% BSA. The sample was analysed immediately.
Abcam response
Thank you so much for testing this antibody in cow.
We appreciate very much your efforts, but are sorry to hear that the antibody did not work well in this species. We would welcome additional feedback from other customers as well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Ina Laura Pieper
Verified customer
提交于 Sep 24 2013