重组生物素Anti-CD11a抗体[EP1285Y] (ab200022)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Biotin Rabbit monoclonal [EP1285Y] to CD11a
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: Biotin
Related conjugates and formulations
概述
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产品名称
生物素Anti-CD11a抗体[EP1285Y]
参阅全部 CD11a 一抗 -
描述
生物素兔单克隆抗体[EP1285Y] to CD11a -
宿主
Rabbit -
偶联物
Biotin -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Cynomolgus monkey -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: normal human spleen tissue.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1285Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)
- Anti-CD11a antibody [EP1285Y] - Low endotoxin, Azide free (ab246701)
- Alexa Fluor® 647 Anti-CD11a antibody [EP1285Y] (ab307264)
- Alexa Fluor® 488 Anti-CD11a antibody [EP1285Y] (ab307764)
- Alexa Fluor® 594 Anti-CD11a antibody [EP1285Y] (ab315256)
- Anti-CD11a antibody [EP1285Y] (ab52895)
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab200022于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. -
组织特异性
Leukocytes. -
序列相似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
结构域
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3683 Human
- Omim: 153370 Human
- SwissProt: P20701 Human
- Unigene: 174103 Human
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别名
- Lymphocyte function associated antigen, type 1, alpha subunit antibody
- Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide antibody
- Antigen CD11A antibody
see all
图片
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IHC image of CD11a staining in a section of formalin-fixed paraffin-embedded normal human spleen*, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab200022 at 1/100 dilution for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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