Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Kidney)
Permeabilization
Yes - SDS 4%
Specification
Kidney
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100
Secondary antibody
Dilution
1/100
Name
Non-Abcam antibody was used: Alexa Fluor 488-AffiniPure Goat Anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Additional data
Additional Notes
IHC image of CD10 staining in Rat normal kidney tissue fixed with PFA 4%, embedded in OTC, 0.5 ´m sections obtained using Leica CM 3050 S system. The section was pre-treated with SDS 4% for 4 mins. The section was then incubated with ab73409 1 ´g/ml dilution overnight at 4¯C and detected using an Alexa fluor 488 conjugated goat antibody. Images were obtained using a Nikon eclipse 90i microscope.
Abcam response
Thank you for sending your valuable feedback. We are pleased to see this has worked in IHC-Fr and rat samples, which are a new application and species.
The customer received an AbTrial testing code to test the antibody. We welcome further feedback from customers who have used the antibody in IHC-Fr or rat which will indicate if the antibody works consistently in this application and species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jul 28 2017