Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Uterus)
Permeabilization
Yes - 0.5% Triton/PBS
Specification
Uterus
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10%serum1%BSAPBS
Dilution
1/100
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
After initial block in 10% serum/PBS, we performed a Mouse-on-Mouse block overnight after which the primary antibody was placed on sample. Sample was co-stained with ZO-2 antibody to label epithelial tissue.
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提交于 Jul 29 2016