Anti-CCR6抗体[MM0066-3L1] (ab93086)
Key features and details
- Mouse monoclonal [MM0066-3L1] to CCR6
- Suitable for: IHC-P, Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG2b
概述
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产品名称
Anti-CCR6抗体[MM0066-3L1]
参阅全部 CCR6 一抗 -
描述
小鼠单克隆抗体[MM0066-3L1] to CCR6 -
宿主
Mouse -
特异性
This antibody detects CCR6, but not other CCRs. -
经测试应用
适用于: IHC-P, Flow Cyt, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human CCR6. 293 cells transfected with Human CCR6.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and 0.2 µm filtered. -
克隆
单克隆 -
克隆编号
MM0066-3L1 -
同种型
IgG2b -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab93086于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration.
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Flow Cyt |
1/100 - 1/800.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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IHC-P |
Use a concentration of 1 µg/ml.
|
说明 |
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IHC-P
Use at an assay dependent concentration. |
Flow Cyt
1/100 - 1/800. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
IHC-P
Use a concentration of 1 µg/ml. |
靶标
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功能
Receptor for a C-C type chemokine. Binds to MIP-3-alpha/LARC and subsequently transduces a signal by increasing the intracellular calcium ions level. -
组织特异性
Spleen, lymph nodes, appendix, and fetal liver. Expressed in lymphocytes, T-cells and B-cells but not in natural killer cells, monocytes or granulocytes. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1235 Human
- Omim: 601835 Human
- SwissProt: P51684 Human
- Unigene: 46468 Human
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别名
- BN 1 antibody
- BN1 antibody
- C C chemokine receptor type 6 antibody
see all
图片
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Overlay histogram showing Jurkat cells stained with ab93086 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab93086, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placental tissue section (4µm) labelling CCR6 with ab93086. Tissue was treated with 15µg/ml Protenase for antigen retrieval and fixed with 4% PFA. A HRP-conjugated anti-mouse IgG was used as the secondary antibody (30 minutes at room temperature) followed by DAB color development.
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IHC image of ab93086 staining in Human Tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93086, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab93086 被引用在 3 文献中.
- Lv S et al. Impaired decidualization caused by downregulation of Circadian Clock Gene BMAL1 contributes to human recurrent miscarriage. Biol Reprod N/A:N/A (2019). PubMed: 30985884
- Dorgham K et al. Identification of the Single Immunodominant Region of the Native Human CC Chemokine Receptor 6 Recognized by Mouse Monoclonal Antibodies. PLoS One 11:e0157740 (2016). PubMed: 27336468
- Tang M et al. Expression and Prognostic Significance of Macrophage Inflammatory Protein-3 Alpha and Cystatin A in Nasopharyngeal Carcinoma. Biomed Res Int 2015:617143 (2015). IHC . PubMed: 26634210