重组Anti-CCR2抗体[EPR20844] (ab216863)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20844] to CCR2
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Mouse, Recombinant fragment
Related conjugates and formulations
概述
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产品名称
Anti-CCR2抗体[EPR20844]
参阅全部 CCR2 一抗 -
描述
兔单克隆抗体[EPR20844] to CCR2 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, Flow Cytmore details -
种属反应性
与反应: Mouse, Recombinant fragment -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- ICC/IF: HEK-293T lysate transfected with GFP-tagged CCR2. Flow Cyt: HEK-293T cells transfected with GFP-tagged CCR2 and mouse primary splenocytes. Mouse bone marrow and spleen
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR20844 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab216863于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/100.
|
|
Flow Cyt |
1/600 - 1/644.
|
说明 |
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ICC/IF
1/100. |
Flow Cyt
1/600 - 1/644. |
靶标
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功能
Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
翻译后修饰
N-glycosylated. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 12772 Mouse
- SwissProt: P51683 Mouse
- Unigene: 6272 Mouse
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别名
- C C chemokine receptor type 2 antibody
- C C CKR 2 antibody
- C-C chemokine receptor type 2 antibody
see all
图片
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Flow cytometry staining of C57 BL/6 mouse splenocytes with ab216863 (right) or Rabbit monoclonal IgG (ab172730) (left). Cells were incubated for 30 min on ice in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab216863 or Rabbit monoclonal IgG (ab172730) (1x 106 in 100 µl at 1.0 μg/ml (1/644)) for 30min on ice. The cells were simultaneously stained with CD11b.
The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min on ice.
Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable cells.
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Flow cytometry staining of C57 BL/6 mouse bone marrow cells with ab216863 (right) or Rabbit monoclonal IgG (ab172730) (left). Cells were incubated for 30 min on ice in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab216863 or Rabbit monoclonal IgG (ab172730) (1x 106 in 100 µl at 1.0 μg/ml (1/624)) for 30min on ice. The cells were simultaneously stained with Ly6G.
The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min on ice.
Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable cells.
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Flow cytometry staining of C57 BL/6 mouse bone marrow cells with ab216863 (right) or Rabbit monoclonal IgG (ab172730) (left). Cells were incubated for 30 min on ice in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab216863 or Rabbit monoclonal IgG (ab172730) (1x 106 in 100 µl at 1.0 μg/ml (1/624)) for 30min on ice. The cells were simultaneously stained with CD11b.
The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min on ice.
Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable cells.
-
Flow cytometry staining of C57 BL/6 mouse splenocytes with ab216863 (right) or Rabbit monoclonal IgG (ab172730) (left). Cells were incubated for 30 min on ice in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab216863 or Rabbit monoclonal IgG (ab172730) 1x 106 in 100 μl at 1.0 μg/ml (1/644)) for 30min on ice. The cells were simultaneously stained with Ly6G. The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min on ice. Acquisition of >30000 events were collected using a n/a. Events were gated on viable cells.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labelling CCR2 with ab216863 at 1/100 dilution, followed by Alexa Fluor® 647 Goat anti-Rabbit secondary (ab150079) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in 293T cells transfected with GFP-tagged CCR2 expression vector. DAPI was used as the nuclear countertsain (Blue).
Secondary antibody only control:
Control 1: Alexa Fluor® 647 Goat anti-Rabbit secondary (ab150079) secondary antibody in GFP transfected HEK-293T cells.
Control 2: Alexa Fluor® 647 Goat anti-Rabbit secondary (ab150079) secondary antibody in CCR2-GFP transfected cells. -
Flow cytometric analysis of HEK-293T (human embryonic kidney epithelial cell) transfected with GFP-tagged CCR2 expression vector, cell line labelling CCR2 with ab216863 at 1/600 dilution (Right) and an isotype control Rabbit monoclonal IgG (ab172730) (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody. Gated on viable cells. Only GFP(+) population give a positive signal.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab216863 被引用在 1 文献中.
- Tian Q et al. Inhibition of CCR2 attenuates neuroinflammation and neuronal apoptosis after subarachnoid hemorrhage through the PI3K/Akt pathway. J Neuroinflammation 19:312 (2022). PubMed: 36566220