Anti-CBF抗体(ab89034)
Key features and details
- Mouse polyclonal to CBF
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-CBF抗体
参阅全部 CBF 一抗 -
描述
小鼠多克隆抗体to CBF -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant full length protein within Human CBF. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- WB: NIH/3T3 cell lysate; K-562 cell lysate; CBF transfected 293T cell lysate ICC/IF: HeLa cell
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab89034于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 121 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 121 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
Stimulates transcription from the HSP70 promoter. -
序列相似性
Belongs to the CBF/MAK21 family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 10153 Human
- Entrez Gene: 12607 Mouse
- Omim: 612828 Human
- SwissProt: Q03701 Human
- SwissProt: P53569 Mouse
- Unigene: 135406 Human
- Unigene: 24169 Mouse
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别名
- CBF antibody
- CBF2 antibody
- CCAAT binding factor antibody
see all
图片
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Anti-CBF antibody (ab89034) at 1 µg/ml + NIH/3T3 cell lysate at 50 µg
Predicted band size: 121 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted? -
Anti-CBF antibody (ab89034) at 1 µg/ml + K-562 cell lysate at 50 µg
Predicted band size: 121 kDa
Observed band size: 131 kDa why is the actual band size different from the predicted? -
All lanes : Anti-CBF antibody (ab89034) at 1 µg/ml
Lane 1 : CEBPZ transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 121 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted? -
ab89034, at 10 µg/ml, staining CBF in Hela cell by Immunofluorescence.
实验方案
数据表及文件
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Datasheet download
文献 (0)
ab89034 尚未被引用在任何文献中。