Anti-CARD15/NOD2抗体(ab115542)
Key features and details
- Rabbit polyclonal to CARD15/NOD2
- Suitable for: WB, IHC-P, ICC
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CARD15/NOD2抗体
参阅全部 CARD15/NOD2 一抗 -
描述
兔多克隆抗体to CARD15/NOD2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICCmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human CARD15/NOD2 (C terminal) conjugated to keyhole limpet haemocyanin.
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阳性对照
- Human small intestine tissue; HeLa cell lysate; HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab115542于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 115 kDa.
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IHC-P |
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC |
Use a concentration of 10 µg/ml.
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说明 |
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WB
Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 115 kDa. |
IHC-P
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC
Use a concentration of 10 µg/ml. |
靶标
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功能
Induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). -
组织特异性
Monocytes-specific. -
疾病相关
Defects in NOD2 are the cause of Blau syndrome (BS) [MIM:186580]. BS is a rare autosomal dominant disorder characterized by early-onset granulomatous arthritis, uveitis and skin rash.
Defects in NOD2 are a cause of susceptibility to inflammatory bowel disease type 1 (IBD1) [MIM:266600]. IBD1 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
Defects in NOD2 are the cause of sarcoidosis early-onset (EOS) [MIM:609464]. EOS is a form of sarcoidosis manifesting in children younger than 4 years of age. Sarcoidosis is an idiopathic, systemic, inflammatory disease characterized by the formation of immune granulomas in involved organs. Granulomas predominantly invade the lungs and the lymphatic system, but also skin, liver, spleen, eyes and other organs may be involved. Early-onset sarcoidosis is quite rare and has a distinct triad of skin, joint and eye disorders, without apparent pulmonary involvement. Compared with an asymptomatic and sometimes naturally disappearing course of the disease in older children, early-onset sarcoidosis is progressive and in many cases causes severe complications, such as blindness, joint destruction and visceral involvement. -
序列相似性
Contains 2 CARD domains.
Contains 9 LRR (leucine-rich) repeats.
Contains 1 NACHT domain. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 64127 Human
- Omim: 605956 Human
- SwissProt: Q9HC29 Human
- Unigene: 592072 Human
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别名
- ACUG antibody
- Arthrocutaneouveal granulomatosis antibody
- BLAU antibody
see all
图片
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Lane 1 : Anti-CARD15/NOD2 antibody (ab115542) at 2 µg/ml
Lane 2 : Anti-CARD15/NOD2 antibody (ab115542) at 4 µg/ml
All lanes : HeLa cell lysate
Predicted band size: 115 kDa -
ab115542, at 3µg/ml, staining CARD15/NOD2 in Formalin-fixed, Paraffin-embedded Human Small intestine tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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ab115542 at 10µg/ml staining CARD15/NOD2 in HeLa cells by Immunocytochemistry.
实验方案
数据表及文件
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Datasheet download
文献 (0)
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