Application
Western blot
Sample
Zebrafish Tissue lysate - whole (zf embryo)
Loading amount
30 µg
Specification
zf embryo
Treatment
de-yolked
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 1% BSA in TBS-T
Secondary antibody
Name
Non-Abcam antibody was used: goat-anti rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 50, 55 kDa
Additional data
Abcam response
Thank you very much for your abreview. This antibody has not previously been tested in western blot or on Zebrafish. Therefore, it is currently unclear whether ab59424 doesn't work well in the application, the species or both. Abcam would welcome feedback from other customers using this antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Gemma Lace
Verified customer
提交于 Jan 15 2010