Application
Western blot
Sample
Human Cell lysate - whole cell (huh-7 cells)
Loading amount
10000 cells
Specification
huh-7 cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Concentration
0.5 µg/ml
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C
Secondary antibody
Name
Non-Abcam antibody was used: ecl sheep anti-rabbit IgG
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
SuperSignal West Pico Substrate
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 60 kDa
Additional data
Additional Notes
Size appears slightly larger than the expected 55 kDa.
Image includes band indicating 100 kDa loading control.
Image includes band indicating 100 kDa loading control.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jun 26 2008