概述

  • 产品名称
    Anti-C6ORF173抗体
    参阅全部 C6ORF173 一抗
  • 描述
    兔多克隆抗体to C6ORF173
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to a region of Human C6ORF173

  • 阳性对照
    • HeLa cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab75827 is purified by a peptide affinity chromatography method.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab75827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation (By similarity). The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres (By similarity). Part of a nucleosome-associated complex that binds specifically to histone H3-containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. CENPW has a fundamental role in kinetochore assembly and function. It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome organization and normal progress through mitosis.
  • 组织特异性
    Highly expressed in ovary, liver, lung and pancreas and to a lower extent in breast and gastrointestinal tract cancers; such as those of the colon, rectum and stomach. Overexpressed in high grade breast invasive tumors. Expressed in many cancer cell types.
  • 序列相似性
    Belongs to the CENPW family.
  • 细胞定位
    Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Nucleus matrix. Nucleus > nucleolus. Constitutively localizes to centromeres throughout the cell cycle, and to the inner kinetochore during mitosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • cancer upregulated gene 2 antibody
    • Cancer-up-regulated gene 2 protein antibody
    • CENP-W antibody
    • cenpw antibody
    • CENPW_HUMAN antibody
    • Centromere protein W antibody
    • chromosome 6 open reading frame 173 antibody
    • CUG2 antibody
    • hypothetical protein LOC387103 antibody
    see all

图片

  • Anti-C6ORF173 antibody (ab75827) at 1 µg/ml + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 10 kDa
    Observed band size : 10 kDa

实验方案

文献

This product has been referenced in:
  • Prendergast L  et al. Premitotic Assembly of Human CENPs -T and -W Switches Centromeric Chromatin to a Mitotic State. PLoS Biol 9:e1001082 (2011). WB ; Human . Read more (PubMed: 21695110) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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