Anti-C5 / C5a抗体[557] (ab11876)
Key features and details
- Mouse monoclonal [557] to C5 / C5a
- Suitable for: WB, Inhibition Assay, Functional Studies, ELISA
- Reacts with: Human
- Isotype: IgG2a
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-C5 / C5a抗体[557]
参阅全部 C5 / C5a 一抗 -
描述
小鼠单克隆抗体[557] to C5 / C5a -
宿主
Mouse -
特异性
This antibody reacts with an epitope on C5 and C5a. The antibody 557 is capable to inhibit the binding of C5a to the C5a receptor through a competitive mechanism, it does not block the cleavage of C5 into C5a and C5b. -
经测试应用
适用于: WB, Inhibition Assay, Functional Studies, ELISAmore details -
种属反应性
与反应: Human -
免疫原
Other Immunogen Type corresponding to Human C5/ C5a.
Database link: P01031 -
表位
Ab11876 recognizes an epitope located within the alpha-chain of C5 that corresponds to C5a. -
阳性对照
- Recombinant C5
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
0.2 µm filtered -
克隆
单克隆 -
克隆编号
557 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab11876于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration.
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Inhibition Assay |
Use at an assay dependent concentration.
|
|
Functional Studies |
Use at an assay dependent concentration.
|
|
ELISA | (1) |
Use at an assay dependent concentration.
|
说明 |
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WB
Use at an assay dependent concentration. |
Inhibition Assay
Use at an assay dependent concentration. |
Functional Studies
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
靶标
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功能
Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis). -
疾病相关
Complement component 5 deficiency
An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705). -
序列相似性
Contains 1 anaphylatoxin-like domain.
Contains 1 NTR domain. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 727 Human
- Omim: 120900 Human
- SwissProt: P01031 Human
- Unigene: 494997 Human
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别名
- Anaphylatoxin C5a analog antibody
- C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 antibody
- C5 antibody
see all
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab11876 被引用在 6 文献中.
- Garcia-Arguinzonis M et al. Alternative C3 Complement System: Lipids and Atherosclerosis. Int J Mol Sci 22:N/A (2021). PubMed: 34066088
- Peng L et al. A Novel Cleavage Pattern of Complement C5 Induced by Chlamydia trachomatis Infection via the Chlamydial Protease CPAF. Front Cell Infect Microbiol 11:732163 (2021). PubMed: 35087765
- Yuan K et al. Complement C3 overexpression activates JAK2/STAT3 pathway and correlates with gastric cancer progression. J Exp Clin Cancer Res 39:9 (2020). PubMed: 31928530
- Lu W et al. C5a aggravates dysfunction of the articular cartilage and synovial fluid in rats with knee joint immobilization. Mol Med Rep 18:2110-2116 (2018). PubMed: 29956782
- Wangdi T et al. The Vi capsular polysaccharide enables Salmonella enterica serovar typhi to evade microbe-guided neutrophil chemotaxis. PLoS Pathog 10:e1004306 (2014). Mouse, Human . PubMed: 25101794
- Kola A et al. Epitope mapping of a C5a neutralizing mAb using a combined approach of phage display, synthetic peptides and site-directed mutagenesis. Immunotechnology 2:115-26 (1996). PubMed: 9373320