Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Diseased mouse kidney | paraffin sections)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization
No
Specification
Diseased mouse kidney | paraffin sections
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Formaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/10
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: Jackson ImmunoResearch
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3
Additional data
Additional Notes
The staining is quite good however, at a dilution of 1:10, a single vial provided enough antibody to stain only ˜5 slides
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Theodore Kaplan
Verified customer
提交于 Dec 21 2016