Application
Western blot
Sample
Mouse Cell lysate - whole cell (fibrotic liver)
Loading amount
20 µg
Specification
fibrotic liver
Gel Running Conditions
Reduced Denaturing (SDS boiling for 5 min)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Other product details
Dilution
1/100
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.03% TBST with 3% BSA
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: ~120 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Hao Wu
Verified customer
提交于 Nov 14 2012