Anti-C2抗体(ab77498)
Key features and details
- Rabbit polyclonal to C2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-C2抗体
参阅全部 C2 一抗 -
描述
兔多克隆抗体to C2 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human C2 aa 500-600 conjugated to keyhole limpet haemocyanin.
(Peptide available asab94384) -
阳性对照
- This antibody gave a positive signal in Human Plasma Total Protein Preparation.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77498于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 1 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 83 kDa).
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说明 |
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IHC-P
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 83 kDa). |
靶标
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功能
Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor 4b to generate the C3 or C5 convertase. -
疾病相关
Defects in C2 are the cause of complement component 2 deficiency (C2D) [MIM:217000]. A deficiency of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus erythematosus. Skin and joint manifestations are common and renal disease is relatively rare. Patients with complement component 2 deficiency are also reported to have recurrent or invasive infections. -
序列相似性
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 3 Sushi (CCP/SCR) domains.
Contains 1 VWFA domain. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 717 Human
- Omim: 217000 Human
- SwissProt: P06681 Human
- Unigene: 408903 Human
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别名
- ARMD14 antibody
- C2 antibody
- C3/C5 convertase antibody
see all
图片
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Anti-C2 antibody (ab77498) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
C2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Furthermore, the 105 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies this target. -
IHC image of Ab77498 staining in Human Liver Carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Ab77498, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab77498 尚未被引用在任何文献中。