重组Anti-C1QC抗体[EPR2984Y] (ab75756)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2984Y] to C1QC
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-C1QC抗体[EPR2984Y]
参阅全部 C1QC 一抗 -
描述
兔单克隆抗体[EPR2984Y] to C1QC -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human C1QC aa 150-250. The exact sequence is proprietary.
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阳性对照
- WB: Human plasma lysate; IHC-P: Human liver tissue; Flow Cyt (intra): Human Peripheral Blood Mononuclear Cell (PBMC) cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2984Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75756于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/100.
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WB |
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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Flow Cyt (Intra)
1/100. |
WB
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes. -
疾病相关
Defects in C1QC are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis. -
序列相似性
Contains 1 C1q domain.
Contains 1 collagen-like domain. -
翻译后修饰
O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 714 Human
- Omim: 120575 Human
- SwissProt: P02747 Human
- Unigene: 467753 Human
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别名
- AI385742 antibody
- C1Q C antibody
- C1qc antibody
see all
图片
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ab75756 at 1/100 dilution staining C1q in human liver by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Anti-C1QC antibody [EPR2984Y] (ab75756) at 1/1000 dilution + human plasma lysate at 10 µg
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa -
Flow Cytometry analysis of Human Peripheral Blood Mononuclear Cell (PBMC) cells labeling C1QC with purified ab75756 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 2% Paraformaldehyde and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Left).
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab75756 被引用在 4 文献中.
- Zhai M et al. Identification of Three Significant Genes Associated with Immune Cells Infiltration in Dysfunctional Adipose Tissue-Induced Insulin-Resistance of Obese Patients via Comprehensive Bioinformatics Analysis. Int J Endocrinol 2021:8820089 (2021). PubMed: 33564304
- Kim EN et al. CRP immunodeposition and proteomic analysis in abdominal aortic aneurysm. PLoS One 16:e0245361 (2021). PubMed: 34428207
- Wolf C et al. Janus kinase inhibition in complement component 1 deficiency. J Allergy Clin Immunol 146:1439-1442.e5 (2020). PubMed: 32325142
- Heo SH et al. Fabry disease: characterisation of the plasma proteome pre- and post-enzyme replacement therapy. J Med Genet 54:771-780 (2017). PubMed: 28835480