Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
Sample
Hamster Cell (Chinese Hamster Ovary cells (TREx CHO cells))
Specification
Chinese Hamster Ovary cells (TREx CHO cells)
Permeabilization
Yes - 0.5% Triton
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: 1% BSA PBS
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Sheep FITC
Host species: Sheep
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Sheep
Clonality: Polyclonal
Conjugation: Fluorescein
Dilution
1/100
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Eleni Petsalaki
Verified customer
提交于 Dec 10 2013