Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse embryonic fibroblasts)
Loading amount
15 µg
Specification
mouse embryonic fibroblasts
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: ~0.5% BSA in TBST
Secondary antibody
Name
Non-Abcam antibody was used: Biorad Goat anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL +
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: none kDa Non-specific: 15, 20, 40, 64, 195 kDa
Positive control
Brd4 knockout mice are embryonic lethal, so Brd4 should be expressed in embryonic cells.
Additional data
Additional Notes
Although there is no band at the expected size (~152kDa), the band around ~64kDa may be an alternate isoform.
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jan 29 2013