Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (kidney epithelial and kidney fibroblast)
Loading amount
20 µg
Specification
kidney epithelial and kidney fibroblast
Gel Running Conditions
Reduced Denaturing (10% SDS)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/2000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBS-0.5%Tween
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 66 kDa
Additional data
Additional Notes
Included with the image of the Western Blot (A) is it's corresponding Coomassie-stained gel (B) since our WesternC Precision Standard did not transfer onto our membrane.
Lanes: 1) Dual Color Precision Standard 2) WesternC Precision Standard 3) Vero and 4) CV-1
The antibody cross-reacts with CV-1 lysates, but not with Vero lysates.
Lanes: 1) Dual Color Precision Standard 2) WesternC Precision Standard 3) Vero and 4) CV-1
The antibody cross-reacts with CV-1 lysates, but not with Vero lysates.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Apr 29 2010