Anti-Borealin/CDCA8抗体(ab67126)
Key features and details
- Mouse polyclonal to Borealin/CDCA8
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Borealin/CDCA8抗体
参阅全部 Borealin/CDCA8 一抗 -
描述
小鼠多克隆抗体to Borealin/CDCA8 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Full length native Borealin/CDCA8 (purified) (Human)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab67126于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (2) |
1/500 - 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). |
靶标
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功能
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment. -
序列相似性
Belongs to the borealin family. -
发展阶段
Cell-cycle regulated. Increases during G2/M phase and then reduces after exit from M phase. -
结构域
The C-terminal region (aa 207-280) represents the dimerization motif. -
翻译后修饰
Phosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230.
Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3. -
细胞定位
Nucleus > nucleolus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Chromosome > centromere. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells. - Information by UniProt
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数据库链接
- Entrez Gene: 55143 Human
- Omim: 609977 Human
- SwissProt: Q53HL2 Human
- Unigene: 524571 Human
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别名
- BOR antibody
- BOREA_HUMAN antibody
- Borealin antibody
see all
图片
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All lanes : Anti-Borealin/CDCA8 antibody (ab67126) at 1/500 dilution
Lane 1 : Borealin/CDCA8 transfected 293T cell lysate
Lane 2 : Untransfected 293T cell lysate
Secondary
All lanes : Goat anti mouse IgG (H&L)-HRP conjugate secondary
antibody at 1/2500 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa
实验方案
数据表及文件
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Datasheet download
文献 (2)
ab67126 被引用在 2 文献中.
- Peng R et al. Identification of Core Genes Involved in the Metastasis of Clear Cell Renal Cell Carcinoma. Cancer Manag Res 12:13437-13449 (2020). PubMed: 33408516
- Pike T et al. PKC? switches Aurora B specificity to exit the abscission checkpoint. Nat Commun 7:13853 (2016). WB, ICC/IF . PubMed: 28004745