Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/1000
Secondary antibody
Dilution
1/200
Name
Abcam antibody:Donkey Anti-Goat IgG H&L (Alexa Fluor® 555) preadsorbed
Additional data
Additional Notes
This antibody produces a strong, nuclear-enriched labeling pattern within PF fixed samples. It does not appear to be compatible with MeOH fixation under these conditions.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
提交于 Sep 30 2016