Anti-ABO抗体[BRIC231] (ab33404)
Key features and details
- Mouse monoclonal [BRIC231] to ABO
- Suitable for: Flow Cyt, Agglutination
- Reacts with: Human
- Isotype: IgG1
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-ABO抗体[BRIC231]
参阅全部 ABO 一抗 -
描述
小鼠单克隆抗体[BRIC231] to ABO -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, Agglutinationmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human ABO. Human erythroleukemic cell line (HEL) established from a 30 year old patient with relapsed erythroleukemia following treatment for Hodgkin lymphoma.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: Tris buffer -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
BRIC231 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab33404于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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Agglutination |
Use at an assay dependent concentration.
|
说明 |
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Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Agglutination
Use at an assay dependent concentration. |
靶标
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功能
This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity. -
通路
Protein modification; protein glycosylation. -
序列相似性
Belongs to the glycosyltransferase 6 family. -
结构域
The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis. -
翻译后修饰
The soluble form derives from the membrane form by proteolytic processing. -
细胞定位
Golgi apparatus > Golgi stack membrane. Secreted. Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid. - Information by UniProt
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数据库链接
- Entrez Gene: 28 Human
- Omim: 110300 Human
- SwissProt: P16442 Human
- Unigene: 654423 Human
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别名
- A transferase antibody
- Abo antibody
- B transferase antibody
see all
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab33404 被引用在 3 文献中.
- Purohit S & She JX Multiplex Glycan Bead Array (MGBA ) for High Throughput and High Content Analyses of Glycan-Binding Proteins Including Natural Anti-Glycan Antibodies. Methods Mol Biol 2460:33-44 (2022). PubMed: 34972929
- Purohit S et al. Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins. Nat Commun 9:258 (2018). PubMed: 29343722
- Gao C et al. Carbohydrate Sequence of the Prostate Cancer-associated Antigen F77 Assigned by a Mucin O-Glycome Designer Array. J Biol Chem 289:16462-77 (2014). PubMed: 24753245