Biotinylated山羊抗小鼠IgG (H+L) (Ready to Use) (ab64255)
概述
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产品名称
Biotinylated山羊抗小鼠IgG (H+L) (Ready to Use)
参阅全部 IgG 试剂 -
经测试应用
适用于: Sandwich ELISA, IHC-Pmore details -
常规说明
STAINING PROTOCOL:- Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.
- Add enough drops of an approriate Hydrogen Peroxide Block to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer.
- Perform appropriate pretreatment if required. Wash 3 times in buffer.
- Apply an appropriate Protein Block and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash 1 time in buffer.
- Apply primary antibody and incubate according to manufacturer's protocol.
- Wash 4 times in buffer. Apply Biotinylated Goat anti Mouse IgG (H+L) solution and incubate for 10 minutes at room temperature. Wash 4 times in buffer.
- Apply enzyme labeled streptavidin and incubate according to manufacturer’s protocol.
- Rinse 4 times in buffer. Place slide in appropriate chromogenic substrate and incubate until desired reaction is achieved. Rinse 4 times in buffer.
- Add enough drops of Hematoxylin to cover the section. Incubate for 1 minute.
- Rinse 7-8 times in tap water. Add Mounting Medium to cover the section.
This reagent is intended to be used in a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.
性能
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形式
Liquid -
存放说明
Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.0985% Sodium azide
Constituent: PBS -
Concentration information loading...
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Reagent说明
This reagent is intended to be used in a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen. -
研究领域
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细胞定位
Secreted -
别名
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
see all -
细胞定位
Secreted
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab64255于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Sandwich ELISA |
Use at an assay dependent concentration. PubMed: 23754944
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IHC-P |
Use at an assay dependent dilution.
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说明 |
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Sandwich ELISA
Use at an assay dependent concentration. PubMed: 23754944 |
IHC-P
Use at an assay dependent dilution. |
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (23)
ab64255 被引用在 23 文献中.
- Agas A et al. Hemolytic iron regulation in traumatic brain injury and alcohol use. Alcohol 109:1-12 (2023). PubMed: 36690222
- Cheon SB & Kim WH Upregulation of SLUG expression in canine mammary gland tumors and its prognostic significance. BMC Vet Res 19:112 (2023). PubMed: 37553661
- Powell JE et al. Targeted gene silencing in the nervous system with CRISPR-Cas13. Sci Adv 8:eabk2485 (2022). PubMed: 35044815
- Wang J et al. Identification of a novel circ_0001946/miR-1290/SOX6 ceRNA network in esophageal squamous cell cancer. Thorac Cancer 13:1299-1310 (2022). PubMed: 35411716
- Delessard M et al. Achievement of complete in vitro spermatogenesis in testicular tissues from prepubertal mice exposed to mono- or polychemotherapy. Sci Rep 12:7407 (2022). PubMed: 35523907