Application
Immunocytochemistry
Sample
Human Cultured Cells (HeLa cell)
Permeabilization
Yes - 0.1% PBST, RT 15min
Specification
HeLa cell
Blocking step
CAS-Block (Invitrogen) as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 10% CAS-Block
Dilution
1/200
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: anti-rabbit IgG-cy3
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Yeongmi Cheon
Verified customer
提交于 Aug 07 2017