Application
Western blot
Sample
Mouse Cell lysate - whole cell (aortic smooth muscle cell)
Loading amount
15 µg
Specification
aortic smooth muscle cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: RT°C
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Diluent: 1% milk/ PBST
Secondary antibody
Name
Non-Abcam antibody was used: Bovine anti-goat IgG
Host species: Cow
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Cow
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 45 kDa
Positive control
HUVEC cell lysate, Rat aortic smooth muscle cell lysate
Additional data
Additional Notes
Lane 1: HUVEC lysate (15 ug)
Lane 2: Mouse aortic smooth muscle cell lysate (15 ug)
Lane 3: Rat aortic smooth muscle cell lysate (15 ug)
Lane 2: Mouse aortic smooth muscle cell lysate (15 ug)
Lane 3: Rat aortic smooth muscle cell lysate (15 ug)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Sep 04 2007