Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat aortic smooth muscle cell)
Loading amount
10 µg
Specification
Rat aortic smooth muscle cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6%
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Bovine anti-goat
Host species: Cow
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Cow
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 45 or 46 kDa
Additional data
Abcam response
We are pleased to receive this valuable feedback for Beta Arrestin 2 antibody (ab31294). The blot shows a main specific band at 45kDa. However, there are several extra bands on the blot. There are several isoforms of this protein which may account for these bands. Any non-specific binding can usually be reduced with procedure optimisation, such as reducing the antibody concentration (which is very high in this case), or increasing the blocking time.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jan 03 2007