Application
Western blot
Sample
Human Cell lysate - whole cell (Epithelial OVCA cell line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
100 µg
Treatment
Perifosine
Specification
Epithelial OVCA cell line
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
4 hour(s) and 0 minute(s)
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL Western Blotting Substrate
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 52 kDa Non-specific: 40 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 18 2016